Summary
Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors.
Form
Liquid
Buffer
0.02M Potassium Phosphate (pH7.2), 0.15M Sodium Chloride
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Expression System
Streptomyces avidinii
Purification
This product was prepared from chromatographically purified streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin. No reaction was observed against anti-Avidin.
Conjugation
Horseradish peroxidase(HRP)
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.